Author/Authors :
Wittke، نويسنده , , Stefan and Fliser، نويسنده , , Danilo and Haubitz، نويسنده , , Marion and Bartel، نويسنده , , Sebastian and Krebs، نويسنده , , Ronald and Hausadel، نويسنده , , Frank and Hillmann، نويسنده , , Meike and Golovko، نويسنده , , Igor and Koester، نويسنده , , Peer and Haller، نويسنده , , Hermann and Kaiser، نويسنده , , Thorsten and Mischak، نويسنده , , Harald and Weissinger، نويسنده , , Eva M، نويسنده ,
Abstract :
The on-line coupling of capillary electrophoresis (CE) with electrospray-time-of-flight mass spectrometry (MS) has been used to obtain patterns of peptides and proteins present in the urine of healthy human individuals. This led to the establishment of a “normal urine polypeptide pattern”, consisting of 247 polypeptides, each of which was found in more than 50% of healthy individuals. Applying CE–MS to the analysis of urine of patients with kidney disease revealed differences in polypeptide pattern. Twenty-seven polypeptides were exclusively found in samples of patients. Another 13, present in controls, were missing. These data indicate that CE–MS can be applied as powerful tool in clinical diagnostics.
