Title of article
Human insulin and desamido human insulin isotherms in ethanol–water reversed phase systems
Author/Authors
Zhou، نويسنده , , Y. and Ottens، نويسنده , , M. and Hansen، نويسنده , , E. and van der Wielen، نويسنده , , L.A.M.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
8
From page
141
To page
148
Abstract
The multi-component isotherms for human insulin (HI) and desamido human insulin (dHI) over reversed phase packing (C18) and with 29.8% (w/w) ethanol–water as mobile phase have been determined experimentally. The isotherms of HI in ethanol–water differ from those obtained with the more commonly applied methanol–water and acetonitrile–water mobile phase, as described in this paper. The isotherm exhibits anti Langmuirian behavior and can be very well modeled by an anti Langmuir isotherm presented in this paper. The HI and dHI anti Langmuir isotherm are determined as: q HI = 8.4 C HI + 3 C HI C dHI 1 − 0.05 C HI − 0.14 C dHI + 0.04 C HI C dHI and q dHI = 11.4 C dHI + 2 C HI C dHI 1 − 0.05 C HI − 0.14 C dHI + 0.04 C HI C dHI
Keywords
Human insulin , Desamido human insulin , Adsorption isotherms , Reversed phase chromatography , Anti-Langmuir isotherm , multimerization
Journal title
Journal of Chromatography A
Serial Year
2004
Journal title
Journal of Chromatography A
Record number
1521220
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