Sample preparation including sol–gel immunoaffinity chromatography for determination of bisphenol A in canned beverages, fruits and vegetables

The paper describes the development of a very simple method to prepare samples of canned food (beverages, fruits and vegetables) for the determination of bisphenol A by isocratic HPLC with fluorescence detection. The new sample preparation method makes use of the selectivity of bisphenol A antibodies immobilized in a silica matrix by an inexpensive and simple sol–gel technique. In spite of applying highly complex food matrices, immunoaffinity columns could be used for clean-up of at least 15 real samples. Limits of detection (S/N = 3) ranged from 0.1 ng/ml for beverages to 4.3 ng/g for vegetables.