Title of article
Rapid and sensitive determination of tryptophan, serotonin and psychoactive tryptamines by thin-layer chromatography/fluorescence detection
Author/Authors
Kato، نويسنده , , Noriyuki and Kojima، نويسنده , , Takashi and Yoshiyagawa، نويسنده , , Shinji and Ohta، نويسنده , , Hikoto and Toriba، نويسنده , , Akira and Nishimura، نويسنده , , Hideo and Hayakawa، نويسنده , , Kazuichi، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
5
From page
229
To page
233
Abstract
A rapid, sensitive and selective method for the determination of tryptophan (Trp), serotonin (5-HT) and psychoactive tryptamines (PATs) by thin-layer chromatography (TLC) with fluorescence detection is proposed. These compounds form fluorophores on the developing plate by heating after spraying with sodium hypochlorite, hydrogen peroxide or potassium hexacyanoferrate(III)-sodium hydroxide reagent. Fluorescent spots (vivid blue) were observed by irradiation with ultraviolet light (365 nm). The detection limits of Trp, 5-HT and PATs were in the range from 0.01 μg to 0.06 μg. This method was effectively applied to the detection of confiscated PAT powder and PAT in abusers’ urine samples.
Keywords
TLC , urine , Psychoactive tryptamine , Tryptophan , Serotonin
Journal title
Journal of Chromatography A
Serial Year
2007
Journal title
Journal of Chromatography A
Record number
1522354
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