Title of article
Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products
Author/Authors
Jeurissen، نويسنده , , Suzanne M.F. and Claassen، نويسنده , , Frank W. and Havlik، نويسنده , , Jaroslav and Bouwmans، نويسنده , , Evelien E. and Cnubben، نويسنده , , Nicole H.P. and Sudhِlter، نويسنده , , Ernst J.R. and Rietjens، نويسنده , , Ivonne M.C.M. and van Beek، نويسنده , , Teris A.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
9
From page
81
To page
89
Abstract
An on-line HPLC screening method for detection of inhibitors of human cytochrome P450 1A2 in extracts was developed. HPLC separation of extracts is connected to a continuous methoxyresorufin-O-demethylation (MROD) assay in which recombinant human P450 1A2 converts methoxyresorufin to its fluorescent metabolite resorufin. The system was tested with three P450 1A2 inhibitors, for which minimum detectable amounts (MDA) ranging from 0.7 to 9.5 ng were obtained. Analysis of a kava kava and a basil extract showed that the on-line system is applicable to complex mixtures, since in both extracts, peaks with P450 1A2 inhibiting activity were observed.
Keywords
On-line detection , P450 1A2 inhibitors , Mixture Analysis , Methoxyresorufin-O-demethylase
Journal title
Journal of Chromatography A
Serial Year
2007
Journal title
Journal of Chromatography A
Record number
1523021
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