Title of article :
Analysis of magnolol and honokiol in biological fluids by capillary zone electrophoresis
Author/Authors :
Chen، نويسنده , , Chiu-Ling and Chang، نويسنده , , Po-Ling and Lee، نويسنده , , Shoei-Sheng and Peng، نويسنده , , Fu-Chuo and Kuo، نويسنده , , Chinghua and Chang، نويسنده , , Huan-Tsung، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2007
Abstract :
Capillary zone electrophoresis (CZE) method was used for analysis of magnolol and honokiol. Under the optimized condition, CZE with UV absorption detection provided that the limit of detection was at μM level. To enhance detection sensitivity of magnolol and honokiol, CZE separation system was coupled with a laser-induced fluorescence (LIF) detector for the first time. The limits of detection of magnolol and honokiol were 12 nM (3.20 ng ml−1) and 18 nM (4.79 ng ml−1), respectively, showing that the CZE-LIF system provides greater than 100-fold sensitivity improvements than does the CZE-UV system. The developed method was applied to analyze magnolol and honokiol in spiked human plasma samples, microsome incubation samples as a preliminary demonstration of its potential in pharmacokinetic studies.
Keywords :
Biological fluids , Laser-induced fluorescence detection , Honokiol , Capillary zone electrophoresis , Magnolol
Journal title :
Journal of Chromatography A
Journal title :
Journal of Chromatography A