Isolation of quercetin-3-O-l-rhamnoside from Acer truncatum Bunge by high-speed counter-current chromatography

Preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of quercetin-3-O-l-rhamnoside from the ethyl acetate extract of the leaves of Acer truncatum Bunge using a two-phase-system composed of ethyl acetate–ethanol–water at a volume ratio of 5:1:5 (v/v/v). In a single operation, 41.9 mg of quercetin-3-O-l-rhamnoside was obtained from 366 mg of the crude extract. High-performance liquid chromatography (HPLC) analyses of the CCC fraction revealed that the purity of quercetin-3-O-l-rhamnoside was over 96%. Its structure was identified by MS, 1H NMR and 13C NMR. Quercetin-3-O-l-rhamnoside was obtained from this plant for the first time.