Title of article
A fast, convenient and rugged procedure for characterizing the selectivity of alkyl-silica columns
Author/Authors
Snyder، نويسنده , , L.R. and Maule، نويسنده , , A. and Heebsh، نويسنده , , A. Bravo-Cuellar، نويسنده , , R. and Paulson، نويسنده , , S. and Carrano، نويسنده , , J. and Wrisley، نويسنده , , L. and Chan، نويسنده , , C.C. and Pearson، نويسنده , , N. and Dolan، نويسنده , , J.W. and Gilroy، نويسنده , , J.J.، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
9
From page
49
To page
57
Abstract
Previous work has shown that the selectivity of reversed-phase columns for HPLC can be described by means of five column parameters: H (hydrophobicity), S* (steric resistance), A (hydrogen-bond acidity), B (hydrogen-bond basicity) and C (cation-exchange capacity). Values of H, S*, etc. can be determined by carrying out retention measurements for 18 test solutes under standardized conditions. The reproducibility of the latter procedure has been evaluated by comparison testing in four different laboratories and found acceptable. An alternative 10-solute test procedure which is more reproducible and convenient (but somewhat less accurate), requires only 2–3 h per column.
Keywords
Cation-exchange capacity , Selectivity parameters , Hydrophobicity , Hydrogen-bond acidity , Steric resistance , Hydrogen-bond basicity , Column selectivity
Journal title
Journal of Chromatography A
Serial Year
2004
Journal title
Journal of Chromatography A
Record number
1524445
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