Enantioseparation of acidic enantiomers in capillary electrophoresis using a novel single-isomer of positively charged β-cyclodextrin: Mono-6A-N- pentylammonium-6A-deoxy-β-cyclodextrin chloride

The new single-isomer of positively charged β-cyclodextrin, mono-6A-N-pentylammonium-6A-deoxy-β-cyclodextrin chloride (PeAM-β-CD), was employed for the first time for the enantioseparation of anionic and ampholytic analytes by capillary electrophoresis (CE). The synthesis and characterization of PeAM-β-CD were reported. The effect of background electrolyte (BGE) pH and selector concentration on the enantioseparation was investigated. Good separation was obtained at low BGE pH (ca. 5.0–6.0). The effective mobilities of all analytes were found to decrease with increasing CD concentration. PeAM-β-CD proved to be an effective chiral selector for most studied anionic analytes.