A comparative study of void volume markers in immobilized-artificial-membrane and reversed-phase liquid chromatography

A comparative study of 10 void volume marker candidates on C8, C18, and IAM columns was described under several pH (3.0, 4.8, and 7.3) buffer conditions. Pycnometric analysis has been performed on the columns for comparison. In acidic conditions, small organic carboxylic acids suggested by the manufacturer did not necessarily give reliable and consistent estimates of the void volume for IAM column. However, changes in temperature and organic modifier composition led to small and predictable effects. The results presented here provide minimal but practical guidelines for the use of void volume markers in IAM chromatography.