Simultaneous determination of endocrine-disrupting phenols and steroid estrogens in sediment by gas chromatography–mass spectrometry

A simple and effective method has been developed to simultaneously determine endocrine-disrupting phenolic xenoestrogens and steroid estrogens in sediment by using ultra-sonicated extraction in combination with silica gel fractionation, derivatization with pentafluropropionic anhydride, and gas chromatography–mass spectrometry (GC–MS) in selected ion monitoring mode (SIM). Satisfactory recoveries have been obtained for phenolic xenoestrogens and steroid estrogens. The method enables the determination of targets at concentrations of lower nanogram-per-gram in sediments. The method has been successfully applied to the sediments collected from Pearl River Estuary (PRE), South China Sea, China. Nonylphenol and bisphenol-A (BPA) were detected in the range from 204.2 to 664.5 ng/g and 0.6 to 4.0 ng/g, respectively. None of the estrogens were found in the sediment samples.