Title of article :
Characterization of cephalosporin transfer between aqueous and colloidal phases by micellar electrokinetic chromatography
Author/Authors :
Yahya Mrestani، نويسنده , , Yahya and Neubert، نويسنده , , Reinhard، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2000
Abstract :
A new electrokinetic chromatographic method was applied to the determination of the partition coefficient between water and micelle for a group of cephalosporins (cefmetazol, cephradin, cefaclor, ceftazidim, cefodizim, cephapirin, cephalothin and ceftriaxon) using sodium dodecyl sulphate as an anionic surfactant in microemulsion and in micellar systems. In the new method, the running buffer contains both the micelles and the drug, and the injected solution contains the same concentration of micelles as the running buffer but not the drug. The mobility of the drug can be measured from a negative peak recorded the chromatogram. The required parameters for the determination of the capacity factor (μaq and μme are the electrophoretic mobilities of the solutes in the aqueous and the micelle phases, μeff is the effective mobility in the micellar system or in the microemulsion) were measured by the new micellar and microemulsion electrokinetic chromatography technique. Linear log–log relationships were found between both the micelle–water partition coefficient and the capacity factor and the n-octanol–water partition coefficient.
Keywords :
Cephalosporins
Journal title :
Journal of Chromatography A
Journal title :
Journal of Chromatography A