Title of article
Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography
Author/Authors
Gimeno، نويسنده , , E and Castellote، نويسنده , , A.I and Lamuela-Raventَs، نويسنده , , R.M and de la Torre، نويسنده , , M.C and Lَpez-Sabater، نويسنده , , M.C، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2000
Pages
4
From page
251
To page
254
Abstract
A quick and direct method for measuring tocopherols (α, β+γ and δ) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (α-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol–water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%).
Keywords
vitamins , Tocopherols
Journal title
Journal of Chromatography A
Serial Year
2000
Journal title
Journal of Chromatography A
Record number
1527445
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