Models of Metabolism inRhizopus oryzae

A flux analysis of glucose metabolism inRhizopus oryzaewas achieved using [14C-labeled glucose and acetate. The rates of glucose utilization and end product production were estimated, and metabolite pool sizes and specific radioactivities were determined. These data were analysed using a specific radioactivity curve-matching program calledTFLUX. The analysis is consistent with the existence of separate mitochondrial and cytosolic pools of pyruvate, malate and fumarate.