Title of article :
Optimization of alkaline phosphatase gene expression in E. coli
Author/Authors :
Mir Mohammad Sadeghi، H. نويسنده Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Sciences Research Center School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran. , , Rabbani، M. نويسنده Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R.Iran. , , Fazeli، M. S. نويسنده ,
Issue Information :
دوفصلنامه با شماره پیاپی 0 سال 2008
Abstract :
Alkaline phosphatase is an enzyme with widespread use in research and industry such as protein labeling, dephosphorylation of nucleic acids, and enzyme based biosensors. In the present study, alkaline phosphatase gene was inserted into the pET15b vector. The recombinant DNA was then expressed using IPTG as an inducer. The expression of this enzyme was optimized by changing expression conditions such as IPTG concentration, time, and temperature. The obtained results demonstrate that with the expression system used, large quantities of active alkaline phosphatase can be produced in E. coli.
Journal title :
Research in Pharmaceutical Sciences
Journal title :
Research in Pharmaceutical Sciences
