Author/Authors :
Sabet Sarvestani، Fatemeh نويسنده Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran , , Tamadon، Amin نويسنده Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University & Stem Cell and Transgenic Technology Research Center, Shiraz Uni Tamadon, Amin , Koohi Hosseinabadi ، Omid نويسنده Stem cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. , , Mohammadi Nezhad، Saeed نويسنده Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran , , Rahmanifar، Farhad نويسنده Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran , , Jafarzadeh Shirazi، Mohammad Reza نويسنده Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran , , Tanideh، Nader نويسنده , , Moghadam، Ali نويسنده Biotechnology Institute, College of Agriculture, Shiraz University, Shiraz, Iran , , Niazi، Ali نويسنده Biotechnology Institute, College of Agriculture, Shiraz University, Shiraz, Iran ,
Abstract :
Background: RFamide-related peptide-3 (RFRP-3) and kisspeptin (KiSS-1) are known
to respectively inhibit and stimulate gonadotropin releasing hormone (GnRH) and luteinizing
hormone (LH) secretion in rat. The aim of the present study was to evaluate the
relative mRNA expression of RFRP-3 and KiSS-1 in the hypothalamus of pregnant rats.
Materials and Methods: In a randomized controlled experimental study, the exact pregnancy
day of 18 Sprague-Dawley rats were confirmed using the vaginal smear method
and were equally assigned to three groups of days 7, 14 and 21 of pregnancy. Four nonpregnant
female rats were ovariectomized and assigned as the control group. All rats
were decapitated, and the dorsomedial hypothalamic nucleus (DMH) and the arcuate
nucleus (ARC) for detection of KiSS-1 mRNA were separated from their hypothalamus
to detect RFRP-3 and KiSS-1 mRNA respectively. Then, their relative expressions were
compared between control and pregnant groups using real-time polymerase chain reaction
(PCR).
Results: The relative expression of RFRP-3 mRNA in DMH did not change significantly
during pregnancy (p > 0.01). However, the relative expression of KiSS-1 mRNA in ARC
was at its highest in day 7 of pregnancy and decreased until day 21 of pregnancy (p < 0.01).
Conclusion: Decrease in GnRH and LH secretion during the pregnancy of rat may be
controlled by constant expression of RFRP-3 mRNA and reduced expression of KiSS-1
mRNA in hypothalamus.
