Interactions of Substrate and Product with Cytochrome P450: P4502B4versus P450cam

In the present study, two P450s (P4502B4and P450cam) have been examined with regard to their interactions with their substrates and products utilizing the characteristic spectral perturbations as criteria for their binding. The results indicate that although there are differences between the two P450s (E) in regard to their precise interactions with their substrates (S) and products (P), the spectral titration data were consistent with the two-site model—E + S ↔ ES (K1), E + P ↔ EP (K2); EP + S ↔ ESP (K3); ES + P ↔ ESP(K4) in which S and P bind to E forming ESP. The data were inconsistent with the two-site model in which S and P compete for the same site. As required by the two-site model, the relationshipK2K3=K1K4was maintained with both P450s at all product concentrations tested, althoughK3andK4decreased considerably when product concentration was increased. The relationshipK3⪢K4was also maintained, indicating that with both enzymesʹ ESP is formed predominantly by binding of S to EP rather than binding of P to ES, and that ESP dissociates predominantly to ES and P rather than EP and S. In other words, binding of S to EP facilitates the dissociation of P. This indicates that the relative parameter values are compatible for ESP to have functional significance. The possible role of ESP in controlling catalytic rate and catalytic efficiency is discussed.