Partitioning of pectinase produced by Polyporus squamosus in aqueous two-phase system polyethylene glycol 4000/crude dextran at different initial pH values

Partitioning of endo-pectinase and exo-pectinase, produced during the cultivation of Polyporus squamosus in an aqueous two-phase system, composed of polyethylene glycol 4000 and crude dextran, at different initial pH values was studied. At all stages cultivation, the biomass produced showed exclusively bottom-sided partition, irrespectively, of the pH. Maximal value of partition coefficient of endo-pectinase, 2.45, was attained on the second day of cultivation at an initial pH 5.0, which was accompanied by a maximal top phase yield of 80.22%. Higher initial pH 7.0 improved the partition coefficient of exo-pectinase about 2.5 times and the top phase yield to 45% on the third day of cultivation in comparison to partition parameters at lower initial pH. P. squamosus did not produce endo-pectinase in aqueous two-phase cultivation at initial pH 7.0.