Safety evaluation of formulations containing carboxymethyl derivatives of starch and chitosan in albino rats

Two composite formulations, based on carboxymethyl derivatives of starch (formulation I) and chitosan (formulation II), used in the preparation of coating formulations to enhance post harvest shelf-life of fruits and vegetables, were evaluated for safety by single dose dietary (formulation I, coating on feed pellet-1.3% w/w and formulation II, coating on feed pellet-1% w/w) and oral (1 ml, 2% aqueous solution) administration to albino rats. Experiment was carried out for 4 weeks. No significant changes were observed in gain in weekly body weight, weight of vital organs and in parameters of haematology and histopathology among experimental groups, thus indicating safety (and non-toxicity) of the coating formulations.