Synthesis of photosensitive luminol releasing compound, luminol-O-2-nitrobenzylate

Luminol-O-2-nitrobenzylate (1), which releases luminol upon light irradiation at 366 nm and can measure light power, was developed. 1 has a 2-nitrobenzyl group at the carbonyl oxygen of luminol. The released luminol emitted light in the presence of NaOH and H2O2. In the concentration range of 0.01–1 μM 1, CL intensities of released luminol increased in proportion to increase of the concentration of 1 with a light irradiation time of 5 min. The CL intensity of 1 increased in proportion to the light power in the range of 450–650 mW/cm2.