Title of article :
Populations having different GalA blocks characteristics are present in commercial pectins which are chemically similar but have different functionalities
Author/Authors :
Guillotin، نويسنده , , S.E. and Bakx، نويسنده , , E.J. and Boulenguer، نويسنده , , P. and Mazoyer، نويسنده , , Edwin J. and Schols، نويسنده , , H.A. and Voragen، نويسنده , , A.G.J.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2005
Pages :
8
From page :
391
To page :
398
Abstract :
Two commercially extracted pectins having different physical properties but similar chemical characteristics were fractionated into sub-populations using ion exchange chromatography. Individual sub-populations were characterised using established strategies (galacturonic acid and neutral sugar content, degree of methyl-esterification) including the use of enzymes (endo- and exo-polygalacturonases) as analytical tool. Some purified populations showed similar degree of methyl-esterification whereas they were eluting at different ionic strength. It was shown that these populations mainly differed in the number of galacturonic acid moieties in ‘endo-polygalacturonase degradable blocks’ and in the location of these blocks within the molecule. The size of the blocks present at the non-reducing end of the pectin was also different within the molecules. The separation of pectins on anion exchanger combined with the use of enzymes allowed us to differentiate pectic sub-populations. Commercial pectins appear to be a mixture of several polymers differing in total charge as well as in the distribution of the charges.
Keywords :
Pectins , anion exchange chromatography , Degree of methyl-esterification , enzymatic degradation , Charge distribution , Degree of blockiness
Journal title :
CARBOHYDRATE POLYMERS
Serial Year :
2005
Journal title :
CARBOHYDRATE POLYMERS
Record number :
1614110
Link To Document :
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