Title of article :
The Oxidative and Nitrosative Chemistry of the Nitric Oxide/Superoxide Reaction in the Presence of Bicarbonate
Author/Authors :
Jourdʹheuil، نويسنده , , David and Miranda، نويسنده , , Katrina M. and Kim، نويسنده , , Sung M. and Espey، نويسنده , , Michael G. and Vodovotz، نويسنده , , Yoram and Laroux، نويسنده , , Stephen and Mai، نويسنده , , Christie T. and Miles، نويسنده , , Allen M. and Grisham، نويسنده , , Matthew B. and Wink، نويسنده , , David A.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1999
Pages :
9
From page :
92
To page :
100
Abstract :
The primary product of the interaction between nitric oxide (NO) and superoxide ([figure]) is peroxynitrite (ONOO−), which is capable of either oxidizing or nitrating various biological substrates. However, it has been shown that excess NO or[figure]can further react with ONOO−to form species which mediate nitrosation. Subsequently, the controlled equilibrium between nitrosative and oxidative chemistry is critically dependent on the flux of NO and[figure]. Since ONOO−reacts not only with NO and[figure]but also with CO2, the effects of bicarbonate ([figure]) on the biphasic oxidation profile of dihydrorhodamine-123 (DHR) and on the nitrosation of both 2,3-diaminonaphthalene and reduced glutathione were examined. Nitric oxide and [figure] were formed with DEA/NO [NaEt2NN(O)NO] and xanthine oxidase, respectively. The presence of [figure] did not alter either the oxidation profile of DHR with varying radical concentrations or the affinity of DHR for the oxidative species. This suggests that the presence of CO2does not affect the scavenging of ONOO−by either NO or[figure]. However, an increase in the rate of DHR oxidation by ONOO−in the presence of [figure] suggests that a CO2-ONOO−adduct does play a role in the interaction of NO or[figure]with a product derived from ONOO−. Further examination of the chemistry revealed that the intermediate that reacts with NO is neither ONOO−norcis-HOONO. It was concluded that NO reacts with bothtrans-HOONO and a CO2adduct of ONOO−to form nitrosating species which have similar oxidation chemistry and reactivity with[figure]and NO.
Journal title :
Archives of Biochemistry and Biophysics
Serial Year :
1999
Journal title :
Archives of Biochemistry and Biophysics
Record number :
1614485
Link To Document :
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