Microwave-assisted solid-phase oligosaccharides synthesis reaction and scavenging activity of synthetic product to free radical

Using phosphoric acid as the catalyst and glucose as substrate, microwave-assisted solid-phase bioactive oligosaccharide synthesis was studied. The optimum reaction conditions were that microwave power output of 800 W, microwave irradiation time 10 min, adding 30% water to initiate reaction, adding 10% heteropoly acid A as the catalyst. The yield ratio of product was 76.56%. The product was characterized with HPLC. The component and proportion of synthetic oligosaccharides included glucose 49.25%, maltose 10.10%, isomaltose 21.78%, maltotriose 2.15%, panose 10.08%, isomaltotriose 5.16%, tetrasaccharide and pentasaccharides 1.61%. adical scavenging test demonstrated that synthetic oligosaccharides possessed property of scavenging oxygen and hydroxyl free radical.