Title of article :
A Highly Conserved 3-Methylhistidine Modification Is Absent in Yeast Actin
Author/Authors :
Kalhor، نويسنده , , Hamid R. and Niewmierzycka، نويسنده , , Agnieszka and Faull، نويسنده , , Kym F. and Yao، نويسنده , , Xiaoyi and Grade، نويسنده , , Stephanie and Clarke، نويسنده , , Steven M. Rubenstein، نويسنده , , Peter A.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1999
Abstract :
To identify a protein histidine methyltransferase from Saccharomyces cerevisiae, we examined purified actin for the presence of the highly conserved 3-methylhistidine residue at position 73 by amino acid analysis of the whole protein and by amino acid analysis and mass spectrometry of the corresponding tryptic fragment. Surprisingly, we found that His-73 is not modified. A similar lack of modification was also found in actin from the yeast Candida albicans, while rabbit muscle actin revealed the expected 3-methylhistidine residue. Phylogenetic analysis of actin sequences suggests that this modification was introduced in evolution after the divergence of yeast from higher eukaryotic organisms, including unicellular eukaryotes such as Acanthamoeba, Dictyostelium, and Physarum, whose actins contain 3-methylhistidine. Our methodology for the analytical determination of 3-methylhistidine in actin offers an improved approach for investigating histidine methylation in proteins.
Journal title :
Archives of Biochemistry and Biophysics
Journal title :
Archives of Biochemistry and Biophysics