Title of article
Role of Interaction between Two Silkworm RecA Homologs in Homologous DNA Pairing
Author/Authors
Kusakabe، نويسنده , , Takahiro and Kawaguchi، نويسنده , , Yutaka and Maeda، نويسنده , , Takuji and Koga، نويسنده , , Katsumi، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2001
Pages
6
From page
39
To page
44
Abstract
Recombinant BmRad51 and BmDmc1, silkworm homologs of the Escherichia coli RecA proteins catalyzing the homologous DNA pairing, were purified from E. coli cells carrying expression vectors. These possessed different enzymatic properties in the joint molecule formation between single-stranded circular DNA and homologous linear double-stranded DNA. The requirement of single-stranded circular DNA for the efficient reaction was twofold higher in BmRad51 than in BmDmc1. Although able to mediate the joint molecule formation independently, a complex of the two enzymes formed prior to single-stranded DNA binding was found to have augmented efficiency of the pairing reaction.
Keywords
dmc1 , meiotic recombination , Bombyx mori , homologous DNA pairing , Rad51
Journal title
Archives of Biochemistry and Biophysics
Serial Year
2001
Journal title
Archives of Biochemistry and Biophysics
Record number
1617824
Link To Document