Temperature-Jump Relaxation Kinetics of Substrate-Induced Spin-State Transition in Cytochrome P450 (Comparison of the Wild-Type and C334A Mutant P450CAM and P4502B4)

The kinetics of binding of the substrate camphor to the cytochrome P450CAM and the C334A mutant as well as the kinetics of binding of benzphetamine to the wild-type P4502B4 have been studied by the temperature-jump relaxation technique in order to distinguish between the two models for substrate-induced spin-state transition. These models are the bimolecular model in which spin-state transition occurs in parallel with substrate binding, and the two-step spin-equilibrium model in which substrate binding is a separate step preceding the spin-state transition. With all three P450s, the relaxation rate versus concentration data were linear as predicted by the bimolecular model and inconsistent with the spin-equilibrium model, which predicts a curve reaching saturation. With all three P450s, the relaxation rate versus concentration data exhibited maxima. These results are considered to resolve the controversy in favor of the bimolecular model for substrate-induced spin-state transition. In addition, the results suggest that the bimolecular model may be applicable to other P450s as well.