Optimization of fermentation conditions for pullulan production by Aureobasidium pullulan using response surface methodology

In this study, response surface methodology was used for optimization the fermentation conditions for pullulan production by the strain Aureobasidium pullulans SK1002 in shaking flask cultures. The production of pullulan was significantly affected by temperature, fermentation time and initial pH. The optimal cultivation conditions stimulating the maximal pullulan production were as follows: temperature, 28 °C; fermentation time, 5 days and initial pH, 5.5. Under these optimized conditions, the predicted maximal pullulan yield was 30.28 g/L. The application of response surface methodology resulted in a significant enhancement in pullulan production. Results of these experiments indicated that response surface methodology was a promising method for optimization of pullulan production.