Effects of sulfated lentinan on cellular infectivity of avian infectious bronchitis virus

In order to investigate the possibility of sulfating modification in improving the anti-viral activity of lentinan (LNT), three kinds of sulfated LNTs (sLNT), sLNT1, sLNT2 and sLNT3 with the degree of substitution of 0.69, 0.98 and 1.37 were prepared by chlorosulfonic acid–pyridine method with three modification condition, respectively. The sLNTs and avain infectious bronchitis virus (IBV) were added into chicken embryo fibroblast (CEF) in three manners, pre-adding polysaccharide, post-adding polysaccharide and mixed adding polysaccharide with IBV, respectively, taking non-modified LNT as control. The anti-viral activities of three sLNTs were compared by MTT method. The results showed that all of sLNTs and LNT at a certain concentration could significantly inhibit IBV growth in three manners. The effects of modified sLNTs were better than that of non-modified LNT. It indicated that sulfated modification could enhance the anti-viral activity of LNT.