Author/Authors :
Cruz، نويسنده , , Ana Katarina Menezes and Andrade، نويسنده , , Giulianna Paiva Viana and Chavante، نويسنده , , Suely Ferreira and de Vasconcelos، نويسنده , , Clلudio L. and Garcia، نويسنده , , Rosângela Balaban and Leite، نويسنده , , Edda Lisboa and Valente، نويسنده , , Ana Paula and Sales، نويسنده , , Mauricio Pereira and Oliveira، نويسنده , , Fernanda Wander، نويسنده ,
Abstract :
A polysaccharide fraction containing acidic galactan (AG) was obtained from eggs of the mollusc Pomacea lineata by precipitation with acetone. Its structure was investigated using a combination of chemical analysis, intrinsic viscosity and NMR spectroscopy methods, including 1D and 2D, COSY, TOCSY and HSQC experiments. The chemistry analysis showed that the acidic galactan did not possess neither sulfate nor uronic acid. The intrinsic and relative viscosities were 0.44 ± 0.05 and 1.744 ± 0.07 dL.g−1, respectively. The NMR confirmed that the acidic galactan had a backbone containing β-d-Gal is a predominant compound and presence of the β-d-GlcNAc in less proportion. In addition, were found pyruvate groups forming six-membered cyclic ketals as 4,6-O-(1′carboxy)-ethylidene-β-d-galactose residues with configuration R in some β-d-Galp.
Keywords :
Mollusc Pomacea lineta , Piruvylated-galactan , VISCOSITY , Acidic galactan , NMR
