• Title of article

    Irradiance dependence of photobleaching of resorufin

  • Author/Authors

    Chang، نويسنده , , Chia-Pin and Nagel، نويسنده , , David J. and Zaghloul، نويسنده , , Mona E. Pedersen، نويسنده ,

  • Issue Information
    روزنامه با شماره پیاپی سال 2011
  • Pages
    3
  • From page
    430
  • To page
    432
  • Abstract
    Resorufin is a fluorescent reporter molecule, the end product in a variety of biochemical analyses. In such assays, excitation sources with high intensity are desirable for fast and precise results. However, high intensity sources bleach resorufin. We report quantitative bleaching rates for resorufin subjected to green excitation over an irradiance range of 5 to about 600 mW/cm2.
  • Keywords
    Optical fluorescence , Photobleaching , Microfluidics , Resorufin
  • Journal title
    Journal of Photochemistry and Photobiology:A:Chemistry
  • Serial Year
    2011
  • Journal title
    Journal of Photochemistry and Photobiology:A:Chemistry
  • Record number

    1621176