Chemoenzymatic synthesis of amylose-grafted alginate and its formation of enzymatic disintegratable beads

This paper reports the chemoenzymatic synthesis of an amylose-grafted alginate. An amine-functionalized maltooligosaccharide was chemically introduced into sodium alginate by condensation with the carboxylates of the alginate to produce a maltooligosaccharide-grafted alginate. Then, a phosphorylase-catalyzed enzymatic polymerization of glucose 1-phosphate from the graft chain ends on the alginate derivative was performed, giving an amylose-grafted alginate. Beads were produced from the product by treatment with CaCl2 aqueous solution, which exhibited disintegratable property by β-amylase. Furthermore, the effect of the enzymatic reaction on the release of an entrapped dye compound from inside of the beads was examined.