Antioxidant activities of different polysaccharide conjugates (CRPs) isolated from the fruiting bodies of Chroogomphis rutilus (Schaeff.: Fr.) O. K. Miller

Four water-soluble purified polysaccharides (CRPsA-1, CRPsA-2, CRPsB-1 and CRPsB-2) were obtained from the fruiting bodies of Chroogomphis rutilus by DEAE Sepharose Fast Flow column chromatography and Sepharose 6 Fast Flow column chromatography. Their chemical and physical characteristics were determined by chemical methods, gas chromatography (GC) and high-performance size-exclusion chromatography (HPSEC). On the basis of hydroxyl radical assay, superoxide radical assay and Fe2+-chelating ability assay, the antioxidant activities of CRPsA-1, CRPsA-2, CRPsB-1 and CRPsB-2 were investigated. Among these fractions, CRPsB-1 and CRPsB-2 containing glucuronic acid had the higher scavenging effects on hydroxyl radicals and superoxide radicals, and more potent Fe2+-chelating activity than CRPsA-1and CRPsA-2 containing no glucuronic acid in a concentration-dependent manner.