Preparation of resistant starch by hydrolysis of maize starch with pullulanase

In this paper, high resistant starch content product was prepared by hydrolyzing of maize starch with pullulanase. The optimal hydrolyzing conditions were investigated and the optimum conditions were as follows: time, 32 h; pH, 5.0; temperature, 46 °C; amount of pullulanase, 12 ASPU/g. The product of resistant starch was obtained by pressure-cooking the resulting hydrolysate in an autoclave at 121 °C for 1 h, cooling at room temperature, storing at 4 °C overnight, autoclaving/cooling for 2 repetition cycle, drying an oven (105 °C) and finally grounding into fine particles (<150 μm). The content of resistant starch in the product was 44.7% (w/w). Results of experiments indicated that this was a promising way of preparation of product of high content of resistant starch.