Author/Authors :
Shen، نويسنده , , Chia-Rui and Juang، نويسنده , , Jyuhn-Huarng and Tsai، نويسنده , , Zei-Tsan and Wu، نويسنده , , Shu-Ting and Tsai، نويسنده , , Fuyuan and Wang، نويسنده , , Jiun-Jie and Liu، نويسنده , , Chao-Lin and Yen، نويسنده , , Tzu-Chen، نويسنده ,
Abstract :
Superparamagnetic iron oxide nanocrystals (SPION) and their colloidal solutions of magnetic nanoparticles (ferrofluid) appear to contribute to the magnetite imaging applications. Here we present an aqueous ferrofluid in physiological pH and its cell tracking potential. In situ coating method was adapted to synthesize N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC)-coated SPION. Characterization results reveal their features as follows: (1) z-average diameter of 274.1 ± 1.934 nm; (2) polydispersity index (PDI) of 0.135 ± 0.029; (3) zeta potential of 11.4 ± 0.410 mV; (4) iron concentration of 5.4 mg Fe/ml; (5) stable in both water and normal saline; (6) uptake by a variety of cell lines and (7) little cytotoxicity. Finally, islet grafting experiments demonstrated the potential usefulness of this product as a MRI contrast agent for cell tracking.
Keywords :
Ferrofluid , Chitosan , islet transplantation , Magnetic resonance image , Superparamagnetic iron oxide nanocrystals (SPION) , Cell tracking
