Extraction optimization of inulinase obtained by solid state fermentation of Aspergillus ficuum JNSP5-06

Production of inulinase by solid state fermentation always involves an extraction step. In this study, the effects of the type of solvent, solid/liquid ratio, stirring rate, temperature, and extraction time on the recovery of inulinase from solid state fermentation of Aspergillus ficuum JNSP5-06 were investigated. After determining the best solvent, the extraction parameters for inulinase recovery were optimized by central composite rotatable design. The results showed that the maximum recovery of inulinase was obtained using 0.1 M sodium acetate of pH 4.5 as extraction solvent, in a solid/liquid ratio of 1:20.5, at 39.6 °C and 207 rpm for 40 min.