Optimization of medium components for hyaluronic acid production by Streptococcus zooepidemicus MTCC 3523 using a statistical approach

Hyaluronic acid (HA), produced from Streptococcus sp., has raised interest in the medical and cosmetics industries because of the various biological functions of HA. Optimization of medium components used for HA production in Streptococcus zooepidemicus MTCC 3523 performed by two-step optimization. A 24 full factorial design was employed to study the effect of crucial factors on HA production. To get optimized levels of these factors further, a central composite design was conducted. Through these two phase experiments carried out for optimization of HA production, the medium that yielded maximum average HA comprises 4.05% of glucose, 5.12% of soyapeptone, 0.075% of MgSO4·7H2O, and 0.25% of K2HPO4 approximately. The maximum average productivity of HA for this composed medium was 0.798 g/L on fermentation in shake flask. The yield of HA was increased by about 65% using these statistical techniques of media optimization.