Production of microbial cellulose: Response surface methodology approach

The present work was carried out to test the potential of Acetobacter aceti MTCC 2623 for the production of cellulose and optimization of various process conditions. Response surface methodology (RSM) was applied to optimize the process parameters for microbial cellulose production. The optimized parameters for maximum cellulose production (1.73 g/L) and sugar utilization (99.8%) obtained were 2.25% (w/v) glucose concentration, 1.16% (w/v) sodium nitrate concentration, pH 7, 27.5 °C temperature, and 159 h of incubation time. The structure of produced microbial cellulose was established by using FT-IR spectroscopy.