Response surface optimized extraction of carbohydrate compound from Folium Ginkgo and its bioactivity

This study aims to investigate the optimization of carbohydrate extraction from Folium Ginkgo. It was found that the optimum conditions for producing carbohydrate compound using response surface methodology (RSM) included a water/solid of 4 and an extraction temperature of 95 °C for 120 min. Fraction-I was a heteropolysaccharide and consisted of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose, with the molar ratio of 1:1.7:2.2:1.8:7.0. Fraction-II also was a heteropolysaccharide and consisted of l-rhamnose, d-mannose, d-glucose and d-galactose, with the molar ratio of 1.8:2.1:1.4:4.3. Then, protective effect of carbohydrate extraction from Folium Ginkgo on oxidative injury in focal cerebral ischemia–reperfusion animals was evaluated in a cerebral ischemia model. Experimental evidence has shown that carbohydrate extraction from Folium Ginkgo have a protective effect against ischemic brain injury.