Author/Authors :
Li، نويسنده , , Congxin and Zhou، نويسنده , , Dezhong and Hu، نويسنده , , Yuling and Zhou، نويسنده , , Hao and Chen، نويسنده , , Jiatong and Zhang، نويسنده , , Zhengpu and Guo، نويسنده , , Tianying، نويسنده ,
Abstract :
The grafting modified chitosan with l-glutathione (GSH) end capped PEG brush-like poly [poly(ethylene glycol) methacrylate] (PMPEG), CS-PMPEG-GSH, as the pDNA condensed vector material could result in a much higher transfection efficiency and lower cytotoxity for NIH3T3 cells. In this work, we have further examined the morphology stabilities of CS-PMPEG-GSH/pDNA vectors at different medium pH values and in the presence of serum protein in detail. And then the targeted characters for HepG2 cells have been probed by tracing the cell uptake behavior and transfection efficiency.
Keywords :
glutathione , Modified chitosan , Non-viral gene vector , Targeted , Nanocomplex
