Author/Authors :
Zong، نويسنده , , Aizhen and Zhao، نويسنده , , Ting and Zhang، نويسنده , , Yan and Song، نويسنده , , Xinlei and Shi، نويسنده , , Yikang and Cao، نويسنده , , Hongzhi and Liu، نويسنده , , Chunhui and Cheng، نويسنده , , Yanna and Qu، نويسنده , , Xianjun and Cao، نويسنده , , Jichao and Wang، نويسنده , , Fengshan، نويسنده ,
Abstract :
A previous study demonstrated that SIP-SII, a sulfated Sepiella maindroni ink polysaccharide, suppressed the invasion and migration of cancer cells via the inhibition of the proteolytic activity of matrix metalloproteinase-2 (MMP-2). Therefore, this study investigated the anti-metastatic effect of SIP-SII in vivo. SIP-SII (15 and 30 mg/kg d) markedly decreased B16F10 pulmonary metastasis in mice models by 85.9% and 88.0%, respectively. Immunohistochemistry showed that SIP-SII decreased the expression of the intercellular adhesion molecule 1 (ICAM-1) and basic fibroblast growth factor (bFGF) in lung metastasis nodules. In addition, SIP-SII inhibited neovascularization in chick chorioallantoic membrane assay at 0.08–2 mg/mL. In the in vitro experiments, SIP-SII (0.8–500 μg/mL) significantly decreased the protein and mRNA expression of ICAM-1 and bFGF in SKOV3 and EA.hy926 cells, respectively. These results suggested that SIP-SII might suppress melanoma metastasis via the inhibition of the tumor adhesion mediated by ICAM-1 and the angiogenesis mediated by bFGF, as well as resulting in depression of the invasion and migration of carcinoma cells.
Keywords :
sulfated polysaccharide , Sepiella maindroni ink , Icam-1 , Angiogenesis , melanoma metastasis