Influence of rutin, FeSO4, Tween 80, aspartate and complex vitamins on synthesis of fungal exopolysaccharide

The influence of several components on exopolysaccharide (EPS) production and antioxidative activity (TEAC, Trolox-Equivalent Antioxidant Capacity) as well as their effects on the morphological development and cell viability of Phellinus sp. P0988 was determined. Rutin, FeSO4, Tween 80 and complex vitamins were found to impose a stronger influence on EPS production and TEAC compared to their effects on the mycelia growth of Phellinus sp. P0988. The relative effects of these components on EPS activity were found to be different from that on EPS yield. Rutin and aspartate significantly affected EPS TEAC (P < 0.05), while FeSO4 and Tween 80 significantly influenced EPS production (P < 0.05). These results yielded the optimum culture medium composition, with an EPS yield and TEAC of 6.2 ± 0.2 g/L and 5.5 ± 0.1 mM, respectively.