Author/Authors :
Andrade، نويسنده , , Giulianna P.V. and Lima، نويسنده , , Marcelo A. and de Souza Junior، نويسنده , , Airton A. and Fareed، نويسنده , , Jawed and Hoppensteadt، نويسنده , , Debra A. and Santos، نويسنده , , Elizeu A. and Chavante، نويسنده , , Suely F. and Oliveira، نويسنده , , Fernanda W. and Rocha، نويسنده , , Hugo A.O. and Nader، نويسنده , , Helena B. Nader، نويسنده ,
Abstract :
A natural heparin-like compound isolated from the crab Goniopsis cruentata was structurally characterized and its anticoagulant and hemorrhagic activities were determined. Enzymatic and nuclear magnetic resonance analysis revealed that its structure is rich in disulfated disaccharides, possessing significant amounts of 2-O-sulfated-β-d-glucuronic acid units. Furthermore, low amounts of trisulfated disaccharide units containing 2-O-sulfated-α-l-iduronic acid were detected, when compared to mammalian heparin. In addition, this heparin-like structure showed negligible in vitro anticoagulant activity and low bleeding potency, facts that make it a suitable candidate for the development of structure-driven, heparin based therapeutic agents with fewer undesirable effects.
Keywords :
heparan sulfate , Nuclear magnetic resonance , Heparinases , Hemorrhagic effect , Anticoagulant activity , HEPARIN
