Author/Authors :
Huang، نويسنده , , Yee and Jiang، نويسنده , , Chunmao and Hu، نويسنده , , Yuanliang and Zhao، نويسنده , , Xiaojuan and Shi، نويسنده , , Cheng and Yu، نويسنده , , Yun and Liu، نويسنده , , Cui and Tao، نويسنده , , Yang and Pan، نويسنده , , Huarong and Feng، نويسنده , , Yibo and Liu، نويسنده , , Jiaguo and Wu، نويسنده , , Yi and Wang، نويسنده , , Deyun، نويسنده ,
Abstract :
The aim of this study is to investigate immunomodulatory effect of rehmannia glutinosa polysaccharide (RGP) on murine splenic lymphocyte and bone marrow derived dendritic cells (DCs). Splenic lymphocytes obtained from mice were co-cultured with RGP for 48 h and then harvested for analyzing with MTT method. The cytokine production of T lymphocytes was measured by ELISA. Effects of RGP treatment on DCs were investigated and assessed by MTT method. The results showed RGP significantly stimulated lymphocyte proliferation and the growth rate of T cell was more significant. The IL-2 and IFN-γ production of T lymphocyte were significantly upregulated after being stimulated with RGP. DCs stimulating on proliferation of T cells and the ability of antigen presenting of DCs have been enhanced under the stimulation of RGP. In conclusion, these findings provided valuable information that RGP possessed strong immunoenhancement activity, which provided the theoretical basis for the further experiment.
Keywords :
Rehmannia glutinosa polysaccharide , dendritic cell , IL-2 , Immunoenhancement , Lymphocyte proliferation , IFN-?
