Author/Authors :
Nobre، نويسنده , , Leonardo Thiago Duarte Barreto and Vidal، نويسنده , , Arthur Anthunes Jacome and Almeida-Lima، نويسنده , , Jailma and Oliveira، نويسنده , , Ruth Medeiros and Paredes-Gamero، نويسنده , , Edgar Jean and Medeiros، نويسنده , , Valquiria Pereira and Trindade، نويسنده , , Edvaldo Silva and Franco، نويسنده , , Celia Regina Cavichiolo and، نويسنده ,
Abstract :
Fucan is a term used to denominate sulfated l-fucose rich polysaccharides. Here, a heterofucan, named fucan B, was extracted from the Spatoglossum schröederi seaweed. This 21.5 kDa galactofucan inhibited CHO-K1 proliferation and migration when fibronectin was the substrate. Fucan B derivatives revealed that such effects depend on their degree of sulfation. Fucan B did not induce cell death, but promoted G1 cell cycle arrest. Western blotting and flow cytometry analysis suggest that fucan B binds to fibronectin and activates integrin, mainly integrin α5β1, which induces FAK/RAS/MEK/ERK activation. FAK activation inhibits CHO-K1 migration on fibronectin and ERK blocks cell cycle progression. This study indicates that fucan B could be applied in developing new antitumor drugs.
Keywords :
fucoidan , cancer , MAPK pathway , antiproliferative
