Author/Authors :
Zhu، نويسنده , , Yang and Li، نويسنده , , Qian and Mao، نويسنده , , Guanghua and Zou، نويسنده , , Ye and Feng، نويسنده , , Weiwei and Zheng، نويسنده , , Daheng and Wang، نويسنده , , Wei and Zhou، نويسنده , , Lulu and Zhang، نويسنده , , Tianxiu and Yang، نويسنده , , Jun and Yang، نويسنده , , Liuqing and Wu، نويسنده , , Xiangyang، نويسنده ,
Abstract :
The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied. In this study, response surface methodology and the Box–Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions. The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03 °C and a time of 33.79 min. The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 ± 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE). The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1. The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes.
Keywords :
Hericium erinaceus polysaccharides , Enzyme-assisted extraction , optimization , characterization , Response surface methodology
