Comparison of Bush Sophora Root polysaccharide and its sulfateʹs anti-duck hepatitis A virus activity and mechanism

In order to research the sulfating modification in enhancing the anti-duck hepatitis A virus (DHAV) activity of Bush Sophora Root polysaccharide (BSRPS), sulfated Bush Sophora Root polysaccharide (sBSRPS) was prepared by chlorosulfonic acid–pyridine method. KBr pellets method was applied to analyze their different structures. Anti-DHAV activity was studied by duck embryonic hepatocytes culture in vitro and artificial inoculation method in vivo. Direct immunofluorescence method and Real-time PCR were applied to study the antiviral mechanism of adsorption, replication and release in vitro and the dynamic change of virus content of blood in vivo. The results showed at the most effective content, sBSRPS (7.813 μg/mL) could inhibit both replication and release of DHAV in vitro, BSRPS (500 μg/mL) only inhibit replication. The relative expression of DHAV gene at the 8th h and the mortality rate of sBSRPS group were significantly reduced. These results indicated sBSRPS performed more effectively in anti-DHAV activity than BSRPS.