Title of article
Characterization of an antiproliferative exopolysaccharide (LHEPS-2) from Lactobacillus helveticus MB2-1
Author/Authors
Li، نويسنده , , Wei and Ji، نويسنده , , Juan and Tang، نويسنده , , Weizhi and Rui، نويسنده , , Xin and Chen، نويسنده , , Xiaohong and Jiang، نويسنده , , Mei and Dong، نويسنده , , Mingsheng، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2014
Pages
7
From page
334
To page
340
Abstract
In this study, we reported that three purified exopolysaccharides fractions (LHEPS-1, LHEPS-2 and LHEPS-3) and crude LHEPS from the Lactobacillus helveticus MB2-1 inhibited the proliferation potential of human gastric cancer BGC-823 cells. We found that all the LHEPS fractions and crude LHEPS exerted concentration and time dependent inhibitory activities in vitro on BGC-823 cells. LHEPS-2 exhibited higher antiproliferative activity against BGC-823 cells in vitro than LHEPS-1, LHEPS-3 and crude LHEPS. With the consideration of the outstanding antiproliferative effect of LHEPS-2 on BGC-823 cells, more fine structural characterization of LHEPS-2 was further identified. The structure of LHEPS-2 was elucidated by methylated analysis, GC–MS, 1H NMR and 13C NMR spectroscopy, together with two-dimensional (2D) COSY, TOCSY, HSQC, HMBC and NOESY spectroscopy. Results showed that the LHEPS-2 was consisted of the following repeating units:
Keywords
Structural characterization , exopolysaccharides , nuclear magnetic resonance spectroscopy , Antiproliferative activity , Lactobacillus helveticus MB2-1
Journal title
CARBOHYDRATE POLYMERS
Serial Year
2014
Journal title
CARBOHYDRATE POLYMERS
Record number
1626156
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