Differential accumulation of Glut1 in the non-DRM domain of the plasma membrane in response to the inhibition of oxidative phosphorylation

Approximately 50% of Glut1 in the plasma membrane of Clone 9 cells is localized to the detergent-resistant membrane (DRM) fraction. Acute exposure (90 min) to 5 mM azide stimulated glucose transport by ∼4.7-fold and increased the abundance of Glut1 in the non-DRM fraction of the plasma membrane by ∼2.9-fold while the abundance of Glut1 in the DRMs was not changed. In parallel experiments, ∼17 h exposure to azide further increased the rate of glucose transport over that observed at 90 min by ∼33% and increased plasma membrane Glut1 content by ∼3.5-fold over control. The increase in total plasma membrane Glut1 reflected a ∼4.7-fold increase of Glut1 content in the non-DRM fraction and a ∼2.6-fold increase in the DRMs. We conclude that acute exposure to azide increases Glut1 content in the non-DRM fractions, while prolonged exposure to azide increases the Glut1 content in both non-DRM and DRM fractions. These changes may play an important role in the stimulation of glucose transport in response to the inhibition of oxidative phosphorylation.