Title of article
12(S)-Hydroperoxyeicosatetraenoic acid (12-HETE) increases mitochondrial nitric oxide by increasing intramitochondrial calcium
Author/Authors
Nazarewicz، نويسنده , , Rafal R. and Zenebe، نويسنده , , Woineshet J. and Parihar، نويسنده , , Arti and Parihar، نويسنده , , Mordhwaj S. and Vaccaro، نويسنده , , Michael and Rink، نويسنده , , Cameron and Sen، نويسنده , , Chandan K. and Ghafourifar، نويسنده , , Pedram، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
7
From page
114
To page
120
Abstract
12(S)-Hydroxyeicosatetraenoic acid (12-HETE) is one of the metabolites of arachidonic acid involved in pathological conditions associated with mitochondria and oxidative stress. The present study tested effects of 12-HETE on mitochondrial functions. In isolated rat heart mitochondria, 12-HETE increases intramitochondrial ionized calcium concentration that stimulates mitochondrial nitric oxide (NO) synthase (mtNOS) activity. mtNOS-derived NO causes mitochondrial dysfunctions by decreasing mitochondrial respiration and transmembrane potential. mtNOS-derived NO also produces peroxynitrite that induces release of cytochrome c and stimulates aggregation of mitochondria. Similarly, in HL-1 cardiac myocytes, 12-HETE increases intramitochondrial calcium and mitochondrial NO, and induces apoptosis. The present study suggests a novel mechanism for 12-HETE toxicity.
Keywords
mtNOS , 12-HETE , Intramitochondrial ionized calcium , mitochondrial transmembrane potential , peroxynitrite , Cytochrome c release , apoptosis , Mitochondrial respiration
Journal title
Archives of Biochemistry and Biophysics
Serial Year
2007
Journal title
Archives of Biochemistry and Biophysics
Record number
1628955
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