Title of article :
Myriocin, an inhibitor of serine palmitoyl transferase, impairs the uptake of transferrin and low-density lipoprotein in mammalian cells
Author/Authors :
Meyer، نويسنده , , Sybille G.E. and Wendt، نويسنده , , Agnieszka E. and Scherer، نويسنده , , Max and Liebisch، نويسنده , , Gerhard and Kerkweg، نويسنده , , Uta and Schmitz، نويسنده , , Gerd and de Groot، نويسنده , , Herbert، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Pages :
9
From page :
60
To page :
68
Abstract :
The role of sphingolipids in clathrin-mediated endocytosis is only poorly understood in mammalian cells. Thus the relationship between sphingolipid de novo synthesis and clathrin-mediated endocytosis of transferrin were studied in L929 fibroblasts and two other cell lines. Endocytosis was measured using live cell imaging with fluorescent transferrin or 125I-transferrin. Lipids were primarily measured using electrospray ionization tandem mass spectrometry. At physiological temperature, transferrin uptake was significantly decreased by the inhibitor of serine palmitoyl transferase myriocin. Myriocin inhibited also the uptake of low-density lipoproteins. The endocytosis inhibition by myriocin could be released by the addition of sphingoid base and by the protein phosphorylation effectors phorbol-12-myristate, 13-acetate (PMA) and okadaic acid. Myriocin influenced not only sphingolipids but also the glycerophospholipid profile. The study of phosphatidylcholine species shows adaptations to more saturated, alkylated and longer fatty acid moieties. The reported results imply that in mammalian cells, at 37 °C, sphingolipid de novo synthesis is required for clathrin-mediated endocytosis.
Keywords :
sphingolipids , Transferrin , low-density lipoprotein , Myriocin , Lipid profiling , Clathrin-Mediated Endocytosis
Journal title :
Archives of Biochemistry and Biophysics
Serial Year :
2012
Journal title :
Archives of Biochemistry and Biophysics
Record number :
1633053
Link To Document :
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